青青久在线视频免费观看_人摸人人人澡人人超碰97首页_99久久久人妻视频_亚欧日韩欧美网站在线看

Honesty is the foundation, the market is changing, and integrity never changes. Welcome to Changsha Meyue BIO Co., Ltd!
Language: 簡體中文簡體中文 EnglishEnglish

Hotline:

400-863-1908

Monday to Friday: 9:00-18:00

Brain mold/heart mold
TTC Staining Solution

TTC Staining Solution

Brief Description:

       2.3.5-Triphenyltetrazolium chloride (TTC) is a lipid soluble photosensitive complex commonly used for detecting seed viability and detecting ischemic stem cells in mammalian tissues. It is the proton

Detailed Introduction

2.3.5-Triphenyltetrazolium chloride (TTC) is a lipid soluble photosensitive complex commonly used for detecting seed viability and detecting ischemic stem cells in mammalian tissues. It is the proton receptor of the pyridine nucleoside structural enzyme system in the respiratory chain, which can react with dehydrogenases in normal tissues and appear red. Dehydrogenases in viable seed embryo tissues and cells can reduce TTC to insoluble red stable triphenylformamide (TTF). If the embryo dies or its vitality declines, it cannot be stained or stained lightly. Therefore, the vitality of seeds can be identified based on the staining site and depth of the embryo.

Technical Parameter

1. Take the brain tissue sample to be tested (usually taken directly after anesthesia or after infusion with physiological saline), and after taking it out, freeze it at 20 ℃ for 20-30 minutes for easy slicing
2. Slice the brain tissue to be examined, with a typical animal sample layer thickness of 0.5-2mm
3. Slice into TTCStain (2%) and immerse in dark for 25-35 minutes
4. Slice and fix in 4% paraformaldehyde or 10% neutral formalin for 4-24 hours
5. Absorb clean water from the surface of the tissue, use image analysis systems such as IPP to measure the area of cerebral infarction and calculate the volume of cerebral infarction


 

Product Consultation

Message Box

  • Product Name:

  • Your Name:

  • Your Phone:

  • Message Content:

Recommended Products

在線客服
Home
QQ
Hotline

Hotline

tel:400-863-1908

WeChat
WeChat
BIN61988
BIN61988
WeChat account has been copied and clicked to enter WeChat